Table 1.
Primers and conditions used in RT-PCR gene expression.
| Gene | Sequence (5′-3′) | Cycles | Product size (bp) | Anneal. temp. °C |
|---|---|---|---|---|
| hTERT | 35 | 408 | 55 | |
| Sense | CGTACATGCGACAGTTCGTG | |||
| Antisense | AGTTCACCACTGTCTTCCGC | |||
|
| ||||
| enJSRV-env | 35 | 1836 | 60 | |
| Sense | ATGCCGAAGCGCCGCGCTGGATT | |||
| Antisense | TCACGGGTCGTCCCCCGCAGCTC | |||
|
| ||||
| syncytin-Rum1 | 35 | 2014 | 60 | |
| Sense | GATGGAGCTGGGTAAGCGAC | |||
| Antisense | TTGTAGCCACGAGTTCCAGG | |||
|
| ||||
| GAPDH | 35 | 300 | 55 | |
| Sense | GTTTGTGATGGGCGTGAACC | |||
| Antisense | CCAGTGAGCTTCCCGTTGAG | |||
RT-PCR: reverse transcriptase polymerase chain reaction; enJSRV-env: endogenous jaagsiekte sheep retrovirus envelope gene; and hTERT: human telomerase reverse transcriptase.