Fig. 2.

GO classification of the DEGs to demonstrate changes in transcript abundance with maturation and mating. GOEAST was used for GO term enrichment analysis, and the relative significant level of enrichment of the differently expressed gene to all genes was calculated with hypergeometric statistical test method, then the GO term whose FDR < 0.001 was considered to be significant enriched. The number of enriched transcripts for GO annotated sequences was revealed in the various samples of different biological categories. a Mature virgin females compared to immature females, and b mated females compared to mature virgin females