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Caffeine-induced inward current. Local puff application of caffeine induced an inward current in control ACSF (red arrow in a) and in the presence of agonist and antagonist of the ryanodine receptor (b, c, and d). Calibrations: 50 pA and 20 s for (a and b); 200 pA and 20 s for (c); and 100 pA and 20 s for (d). Amplitude (e) and duration (f) of caffeine-induced inward currents in response to intracellular application of cADPR, ruthenium red (RR), and ryanodine.
