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Ethidium bromide stained PFGE gel (left panel) and corresponding Southern hybridization blot (right panel) obtained with a PCR-derived, 32P-labeled probe from ESX-region of Mycobacterium abscessus subsp. bolletii strain 5625. Lane 1: M. abscessus subsp. bolletii type strain; lane 2: M. bolletii 5625. Lane 3: Mycobacterium canettii control strain STB-D (described in (Supply et al. 2013)), lane 4: low-range PFG Marker (NEB). PFGE conditions: 5 s ramping to 35 s; 6 V/cm; 23 h.
