Fig. 6.—

Co-integration of a casposon and an unrelated MGE into the same tRNA-Leu gene. (A) Schematic representation of the genomic locus of Methanosarcina mazei strain 1.F.A.1B.3, containing casposon MetMaz1FA1A3-C2 and integrating element MetMaz1FA1A3-E1. The genome map of the latter is also shown with open reading frames being shown as arrows, indicating the direction of transcription. attL and attR, left and right attachment sites, respectively; MTase, methyltransferase; SF2, superfamily 2; Y-Int, integrase of the tyrosine recombinase superfamily. (B) Detailed view of the tRNA-Leu gene (red arrow) region encompassing the attL (green outline) and TSD (light blue shading) sequences as well as a fragment of TIR (dark blue shading).