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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Dec 23;1858(4):783–792. doi: 10.1016/j.bbamem.2015.12.024

Fig. 6.

Fig. 6

Reduction in the hydrophobic volume of all three S5 residues located near Phe-240 fully activates Slo2.1 channels. (A) Irel measured at 0 mV in the absence (control) or presence of 1 mM NFA for L209A channels containing additional Ala substitutions of other S5 residues also predicted to be in close proximity to Phe-240 (n = 7–10). *, indicates an increase (p < 0.01) in both measures of Irel compared to L209A channels. (B) I–Vt relationships for L209A/I210A/C213A Slo2.1 channels in the absence and presence of 1 and 6 mM NFA (n = 6). Oocytes were bathed in KCM211 extracellular solution. (C) I–Vt relationships for L209A/I210A/C213A Slo2.1 channel currents measured for oocytes bathed in K104 extracellular solution in the absence and presence of 1 and 6 mM NFA (n = 4). Error bars indicate + S.E. (A) or ± S.E. (B, C).