Table I.
SIVΔnef RNA in mucosal sites and lymphoid tissues1
| Days post vaccination |
Number of SIV RNA copies 108 CE | |||
|---|---|---|---|---|
| 4 | 7 | 11 | 14 | |
| Pelvic LN | NA | 4.7×107 | NA | NA |
| Inguinal LN | 5.8×105 | 7×106 | NA | 3.6×106 |
| Mesenteric LN | 2.3×105 | NA | 2×106 | 2.9×106 |
| Axillary LN | 4.7×104 | 2×106 | 3×105 | 3.5×105 |
| GALT | 1.1×106 | 1.7×106 | 1.1×105 | 1.9×106 |
| Spleen | 2.5×106 | 4.9×107 | 5.6×106 | 2.3×107 |
| Lung | 4×103 | 2×104 | 1.8×104 | 5.6×104 |
| Cervix | 7.7 × 102 | 1.5 ×106 | 9.3×103 | 7.4×105 |
| Vagina | 1.8×102 | 2.7×105 | 1.9×104 | 1.4×104 |
| Weeks post vaccination | 5 | 5 | 5 | |
| Cervix | 4×103 | 6.8×103 | 2.4×103 | |
| Vagina | NA | NA | 7.9×104 | |
| 20 | 20 | 20 | ||
| Cervix | 6.4×102 | 1.3×102 | 7.9×102 | |
| Vagina | NA | NA | 5.8×104 | |
1
SIV RNA copies per 108 cell equivalents (CE). When sufficient tissue was available for the assay, RNA was extracted from single animals from the tissue sites and days shown following SIVΔnef iv infection. There was sufficient cervical and vaginal tissue available from 3 animals each at 5 and 20 weeks for the assay. Copy numbers in extracted tissue RNA were determined by RT-PCR using specific primers, and expressed as number of copies of SIVΔnef RNA per 108 CE. The values shown for GALT are the highest respectively for jejunum, ileum, colon and rectum. NA, tissue samples of sufficient size for the assay were not available.