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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: J Immunol. 2016 Feb 3;196(6):2572–2582. doi: 10.4049/jimmunol.1501916

Figure 5. YY1 regulates the TCRα repertoire.

Figure 5

Genomic DNA extracted from DP thymocytes from Yy1f/f (WT) and Yy1f/f Lck-Cre (Yy1Lck) mice was analyzed for rearrangement of Vα segments to 5’Jα segments (A) and to 3’Jα segments (B) by real-time PCR with normalization to Cd14. Data represent the mean ± SEM of three DNA preparations for each genotype, each preparation representing a different mouse. (C) Rag1 and Rag2 transcription in DP thymocytes was analyzed by real-time PCR with normalization to Hprt. Data represent the mean ± SEM of three cDNA preparations for each genotype, each preparation representing a different mouse. Statistical significance was evaluated by unpaired Student’s t-test with Holm-Sidak correction for multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001.