. Author manuscript; available in PMC: 2017 Apr 1.

Published in final edited form as: Biochim Biophys Acta. 2016 Jan 30;1858(4):866–871. doi: 10.1016/j.bbamem.2016.01.028

Table 1.

Primers used for site-directed mutagenesis of hTPPT.

Mutation	Forward and Reverse Primers (5′-3′)
N29D	CGAGGCCCCATCAAGGACAGAAGCTGCACAGATGTCATC GATGACATCTGTGCAGCTTCTGTCCTTGATGGGGCCTCG
N69D	CAAGTCCTCTACCCCAGGGACTCTACTGGGGCCTAC GTAGGCCCCAGTAGAGTCCCTGGGGTAGAGGACTTG
N155D	CCAGGGGTACCCTGGGATATGACGGTGATCACAAGC GCTTGTGATCACCGTCATATCCCAGGGTACCCCTGG
N197D	GCGCTCCCAGGGATCACCGATGACACCACCATACAGCAG CTGCTGTATGGTGGTGTCATCGGTGATCCCTGGGAGCGC
N298D	CAGCTGGGTTTCACCACCGACCTCAGTGCCTACCAG CTGGTAGGCACTGAGGTCGGTGGTGAAACCCAGCTG
N393D	GTGCTCTGGGCATCCGACATCAGCTCCCCCGGC GCCGGGGGAGCTGATGTCGGATGCCCAGAGCAC
N409D	CCAATAAATACATCATGCGACCCCACGGCCCACCTTGTG CACAAGGTGGGCCGTGGGGTCGCATGATGTATTTATTGG
N416D	ACGGCCCACCTTGTGGACTCCTCGTGCCCAGGGCTG CAGCCCTGGGCACGAGGAGTCCACAAGGTGGGCCGT

The mutated DNA codon is underlined.