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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Domest Anim Endocrinol. 2015 Oct 31;55:32–40. doi: 10.1016/j.domaniend.2015.10.008

Fig. 4.

Fig. 4

Effect of angiogenin (300 ng/mL; ANG), cortisol (300 ng/mL), prostaglandin E2 (PGE2; 300 ng/mL), Sonic hedgehog (SHH; 500 ng/mL), and wingless-3A (WNT3; 300 ng/mL) on BRB mRNA abundance in IGF1-treated (Control) small-follicle GC of Exp. 4. Cells were cultured for 48 h as described in Materials and Methods, and then treated for 24 h with 30 ng/mL of IGF1 and the indicated hormones for 24 h. Values are means ± SEM of 3 separate experiments. *Asterisk indicates mean differs (P < 0.05) from Control.