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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: J Immunol. 2016 Feb 10;196(6):2838–2846. doi: 10.4049/jimmunol.1500639

Figure 4. High CD45RB expression tunes low affinity primed memory CD8+ T cells for heterologous rechallenge responses.

Figure 4

OT-I T cells were activated in vitro with N4 OVA, Q4 OVA, T4 OVA, or V4 OVA peptide for 4 days. (A) Expression of CD44 and CD45RB on OVA APL Tm cells. (B) V4 OVA APL Tm cells were restimulated for 5 h with N4 OVA, Q4 OVA, T4 OVA, or V4 OVA peptide and Nur77 and CD69 expression was assessed. (C–D) Q4 OVA primed OT-I cells were sorted into CD45RBhi and CD45RBlo populations and adoptively transferred into naïve congenic hosts and rechallenged with N4 OVA peptide (C) The frequency of Q4 OVA cells was assessed 5 days following rechallenge. (D) Expression of CD45RB on Q4 OVA Tm cells before and after rechallenge with N4 OVA peptide for 5 days. Data compiled from 2–3 independent experiments. **p<0.01.