Two groups of B6 mice (n=6) were immunized with IRBP1–20/CFA, then, on day 8 post-immunization, one group was injected i.p. with a single dose of ADA (5U/mouse) and the other with PBS.
(A) A schematic procedure of disease induction and examination
(B) Time-course of the EAU clinical score.
(C) Eye samples from each group were taken on day 25 post-immunization and sections subjected to pathological examination by H&E staining.
(D) Serum was collected on day 13 post-immunization and IL-6, IL-10, IFN-γ, and IL-17 levels measured by ELISA. The data are from a single experiment, representative of three independent experiments **, p < 0.05.