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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: J Immunol. 2016 Feb 15;196(6):2799–2808. doi: 10.4049/jimmunol.1502146

Figure 3.

Figure 3

LincRNA-Cox2 Modulates Transcription of Genes in Response to LPS Stimulation in Macrophages. (A) Quantitative PCR analysis of lincRNA-Cox2 induction in RAW264.7 and BV2 cells treated by two separate siRNAs to lincRNA-Cox2. (B) Heatmaps of representative early-primary, late-primary, and secondary responsive genes in BV2 cells in response to LPS stimulation (4h). The siRNA-A was used to silence lincRNA-Cox2 in cells. (C) LincRNA-Cox2 silencing on LPS-induced expression of Ccl5, Saa3, and Cxcl2 genes in BV2 cells as measured by real-time PCR (LPS for 4h). (D) Overexpression of lincRNA-Cox2 induces Ccl5 expression and attenuates the inhibitory effect of lincRNA-Cox2 siRNA on LPS-induced Ccl5 expression in BV2 cells. Cells were transfected with the full-length of lincRNA-Cox2 or siRNA-A to lincRNA-Cox2 for 24h, exposed to LPS stimulation for 4h and followed by real-time PCR. U2 RNA was measured as a control to rule out non-specific effect of full-length lincRNA-Cox2 transfection. (E) Shift of Saa3 and Ccl5 as early-responsive genes in RAW264.7 cells expressing lincRNA-Cox2. Cells were transfected with the full-length of lincRNA-Cox2 for 24h followed by LPS stimulation for up to 4h. Expression levels of Saa3 and Ccl5 genes were measured by PCR. Data represent mean ± SE from three independent experiments.