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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: J Immunol. 2016 Feb 15;196(6):2799–2808. doi: 10.4049/jimmunol.1502146

Figure 5.

Figure 5

The lincRNA-Cox2/SWI/SNF complex is recruited to the promoter regions of Saa3 and Ccl5 loci in cells in response to LPS stimulation. The lincRNA-Cox2/SWI/SNF complex is recruited to the promoter regions of Saa3 and Ccl5 loci in cells in response to LPS stimulation. (A) ChIP analysis of occupancy of SWI/SNF complex to the promoter region of Saa3 gene in RAW264.7 cells in response to LPS stimulation. Cells were exposed to LPS (1 μg/ml) for 2h followed by ChIP analysis for the occupancy of SWI/SNF complex using anti-Brg1. (B) Inhibition of SWI/SNF occupancy to the Saa3 promoter in response to LPS stimulation by siRNAs to MyBBP1A or lincRNA-Cox2. Cells were transfected with the siRNAs to MyBBP1A or lincRNA-Cox2 for 24h, exposed to LPS for 2h, followed by ChIP analysis for Brg1. (C) Occupancy of SWI/SNF complex to the promoter region of Ccl5 gene and its inhibition by lincRNA-Cox2 knockdown in RAW264.7 cells in response to LPS stimulation. Cells were transfected with the siRNAs to lincRNA-Cox2 for 24h, exposed to LPS for 2h, followed by ChIP analysis for Brg1. (D) ChIRP analysis of occupancy of lincRNA-Cox2 to the promoter region of Saa3 and Ccl5 gene promoters in RAW264.7 cells in response to LPS stimulation. Cells were exposed to LPS (1 μg/ml) for 2h followed by ChIRP analysis for the occupancy of lincRNA-Cox2. Data represent mean ± SE from three independent experiments.