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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: J Immunol. 2016 Feb 5;196(6):2614–2626. doi: 10.4049/jimmunol.1501970

Figure 2.

Figure 2

SPPL3 is required for normal receptor expression and development of NK cells. (A) Representative flow panels of the NK cell gate (Lin−CD122+DX5+) showing the profile of the indicated receptors in the spleen. NK1.1 and DX5 negative controls are unstained cells, others are isotype controls. (B) The percentage of the NK cell gate that is positive for the indicated receptors as depicted in (A). (C) The median fluorescence intensity of the positive gates in (A) of indicated receptors on NK cells, calculated as a percent of the maximum for each receptor. (D) Representative flow panels of the NK cell gate in the indicated organs. (E) The absolute number of each maturation stage in NK cells. Pooled data from three independent experiments with n=3 mice per genotype. Each data point represents one mouse. **p<0.01, ***p<0.001, ****p<0.0001 (unpaired Student’s t-test, statistical significance determined using the Holm-Sidak method, with alpha=5.000%. Each row was analyzed individually, without assuming a consistent SD).