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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: J Immunol. 2016 Feb 5;196(6):2614–2626. doi: 10.4049/jimmunol.1501970

Figure 3.

Figure 3

SPPL3 is required in a cell intrinsic manner for NK cell number and function. (A) Absolute number of NK cells in the spleen and bone marrow from NKp46-iCre mice. Pooled data from three experiments with n=3 mice per genotype. (B) Western blot showing SPPL3 expression in splenic NK cells sorted for Gr-1−DX5+ after NK cell isolation. Representative panel from two experiments with n=4–8 mice per genotype. (C) Percent RMA/s cells (of the total RMA + RMA/s cells collected) remaining 48 hours after injection. Pooled data from three independent experiments with n=2–10 mice per genotype. (D) Percent specific YAC-1 lysis after four-hour co-culture with isolated splenic NK cells (equivalent DX5+ cell number) at 2:1 E:T ratio. Pooled data from three independent experiments with n=3 mice per genotype. (E) Representative flow panels of the NK cell gate in the indicated organs. (F) The absolute number of cells in each maturation stage in NK cells. Pooled data from three independent experiments with n=3 mice per genotype. Each data point represents one mouse, except YAC-1 lysis, where each data point represents the mean. *p<0.05, ***p<0.001, ****p<0.0001 (two-tailed unpaired Student’s t-test with Welch’s correction). For experiments in panel F, statistical significance was determined with an unpaired Student’s t-test using the Holm-Sidak method, with alpha=5.000%. Each row was analyzed individually, without assuming a consistent SD.