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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: J Immunol. 2016 Feb 12;196(6):2450–2455. doi: 10.4049/jimmunol.1502525

Figure 4. Rechallenged RTE-derived memory T cells recapitulate the primary response.

Figure 4

5x103 each of OT-I TCR Tg RTEs and MN T cells were co-transferred into congenic hosts that were infected the following day with Lm.OVA. Mice were then infected with VSV.OVA 66 or 252 days later, as indicated. A) and C) Blood was analyzed prior to VSV.OVA infection. B) and D) At 5 days post secondary infection, blood and splenocytes were analyzed to determine the proportion of RTE- and mature-derived donor cells (left panel), and splenocytes were analyzed for the absolute number (2nd panel) of RTE- and mature-derived T cells and for their IL-2 and IFNγ production after in vitro restimulation (right 2 panels). Data are presented as mean ± SEM and statistical significance assessed using a paired Student’s t test.