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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: J Immunol. 2016 Feb 3;196(6):2742–2752. doi: 10.4049/jimmunol.1502331

Fig. 4. AT-RvD1 dampens production of pro-inflammatory cytokines.

Fig. 4

Levels of pro-inflammatory cytokines were measured in bronchoalveolar lavage fluid of AT-RvD1 (veh- black bars, 20ng/mouse- dark grey bars, or 100ng/mouse- light grey bars, OA) and/or NTHi (1 × 106 CFUs, OA) inoculated mice at 6hrs and 24hrs. Total protein was determined by BCA (A). BALF was assessed for IL-6 (B), TNFα (C), KC (D), and IL-10 (E). PGE2 (F) and Cox-2 (G, H) expression were also evaluated in lung homogenates by EIA and Western Blot, respectively (representative blots shown, densitometry on n=4-5 mice/group). Statistical significance was determined by two-way ANOVA (overall p<0.0001 NTHi [A, B, C, D, F, H], p=0.030 AT-RvD1 [B 6hr], p=0.0257 AT-RvD1 [D 24hr], p=0.0131 AT-RvD1 [H 6hr]) with Bonferroni’s posttest for multiple comparisons (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001), n= 4-6 mice/group.