Table 1.
Plackett-Burman experimental design matrix for screening the cultural medium and conditions with mycelium biomass and yield of crude EPS as response.
| Runs | Experimental factors | Yields of EPS (g/L) | T-AO activity (U/mg) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X 1 | X 2 | A | X 3 | X 4 | B | X 5 | X 6 | C | X 7 | X 8 | |||
| 1 | 1 (10.0) | −1 (5.0) | 1 | −1 (100) | 1 (10.0) | 1 | −1 (120) | −1 (1.30) | −1 | 1 (30) | 1 (96) | 3.634 ± 0.124 | 2.146 ± 0.130 |
| 2 | 1 (10.0) | −1 (5.0) | 1 | −1 (100) | −1 (8.0) | −1 | 1 (150) | 1 (1.63) | 1 | 1 (30) | −1 (72) | 1.956 ± 0.171 | 1.371 ± 0.098 |
| 3 | −1 (8.0) | 1 (6.5) | −1 | −1 (100) | −1 (8.0) | 1 | 1 (150) | −1 (1.30) | 1 | 1 (30) | 1 (96) | 1.724 ± 0.163 | 0.864 ± 0.074 |
| 4 | −1 (8.0) | −1 (5.0) | 1 | 1 (125) | 1 (10.0) | −1 | 1 (150) | −1 (1.30) | 1 | −1 (25) | 1 (96) | 2.135 ± 0.174 | 1.776 ± 0.106 |
| 5 | 1 (10.0) | −1 (5.0) | −1 | 1 (125) | −1 (8.0) | 1 | −1 (120) | 1 (1.63) | 1 | −1 (25) | 1 (96) | 1.481 ± 0.133 | 1.542 ± 0.095 |
| 6 | −1 (8.0) | −1 (5.0) | −1 | 1 (125) | 1 (10.0) | 1 | 1 (150) | 1 (1.63) | −1 | 1 (30) | −1 (72) | 1.519 ± 0.147 | 1.557 ± 0.107 |
| 7 | 1 (10.0) | 1 (6.5) | −1 | −1 (100) | 1 (10.0) | −1 | 1 (150) | 1 (1.63) | −1 | −1 (25) | 1 (96) | 0.935 ± 0.131 | 0.785 ± 0.034 |
| 8 | 1 (10.0) | 1 (6.5) | −1 | 1 (125) | 1 (10.0) | −1 | −1 (120) | −1 (1.3) | 1 | 1 (30) | −1 (72) | 2.493 ± 0.195 | 2.217 ± 0.112 |
| 9 | −1 (8.0) | −1 (5.0) | −1 | −1 (100) | −1 (8.0) | −1 | −1 (120) | −1 (1.30) | −1 | −1 (25) | −1 (72) | 2.061 ± 0.144 | 1.563 ± 0.083 |
| 10 | 1 (10.0) | 1 (6.5) | 1 | 1 (125) | −1 (8.0) | 1 | 1 (150) | −1 (1.30) | −1 | −1 (25) | −1 (72) | 1.664 ± 0.209 | 1.782 ± 0.091 |
| 11 | −1 (8.0) | 1 (6.5) | 1 | −1 (100) | 1 (10.0) | 1 | −1 (120) | 1 (1.63) | 1 | −1 (25) | −1 (72) | 0.091 ± 0.010 | 0.637 ± 0.047 |
| 12 | −1 (8.0) | 1 (6.5) | 1 | 1 (125) | −1 (8.0) | −1 | −1 (120) | 1 (1.63) | −1 | 1 (30) | 1 (96) | 0.704 ± 0.017 | 0.345 ± 0.022 |
Notes: X 1, solid-to-liquid ratio (%, m/v); X 2, bran powder content (%); X 3, volume of liquid medium (mL); X 4, inoculation amount of mycelium (%); X 5, rotation rate (rpm); X 6, concentration of glacial acetic acid (%); X 7, cultural temperature (°C); X 8, cultivation time (h); A, B, and C refer to dummy columns.