Figure 2.

Example flow cytometry dot plots to display the gating and designation of CD25^hi cells among PBMC lymphocytes. CD25^hi events were gated on the basis that expression is of higher intensity in CD4⁺ events (A). Among gated CD4⁺ events, the CD25^hi population contains FOXP3⁺ events (B), IL-10⁺ events (C), and corresponds to the CD127^lo population (D). Among gated CD4⁺CD25^hi events, the majority of IL-10⁺ events were also FOXP3⁺ (E).