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. 2016 Mar 7;7:71. doi: 10.3389/fimmu.2016.00071

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Copyright © 2016 Hussain, Letley, Greenaway, Kenefeck, Winter, Tomlinson, Rhead, Staples, Kaneko, Atherton and Robinson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Influence of recombinant IL-10 and TGFβ or their blocking antibodies on the in vitro IgE response of PBMCs from H. pylori-infected and -uninfected donors. PBMCs from 5 infected and 10 uninfected donors were cultured for 12 days under Th2-skewing conditions, with the addition of rIL-10 (A), rTGFβ (B), anti-IL-10 (C), or anti-TGFβ (D) blocking monoclonal antibodies. Equivalent volumes of medium were added to further wells as controls for recombinant cytokines; equivalent concentrations of isotype control antibodies were added as controls for the cytokine-blocking antibodies. Total IgE concentrations were assayed by ELISA and the percentage change was calculated for each individual with respect to the control cultures. Points represent the results from individuals and horizontal lines depict the median.