Table 3.
Cell viability of MCF-7:5C cells treated with E2 and test compounds
|
|||||
|---|---|---|---|---|---|
|
| |||||
| Compounds | X | R | Inhibition of cell growth relative to E2 %a |
||
| 1 nM | 10 nM | 100 nM | |||
| 3 | NA | NA | NA | ||
| 14a | -OH |
|
11.5 ± 4.0 | 60.2 ± 2.7 | 109.0 ± 1.4 |
| 14b | -F |
|
55.6 ± 3.5 | 69.7 ± 3.6 | 108.0 ± 2.6 |
| 14c | -Cl |
|
NA | NA | NA |
| 19 | -F |
|
NA | NA | NA |
| 21a | -F |
|
NA | NA | 18.9 ± 2.8 |
| 21b | -F |
|
95.4 ± 2.7 | 99.8 ± 2.8 | 100.0 ± 2.1 |
| 21c | -F |
|
4.1 ± 3.6 | 15.1 ± 3.1 | 110.9 ± 4.6 |
| 21d | -F |
|
13.3 ± 3.8 | 7.4 ± 4.8 | 110.0 ± 4.6 |
| 21e | -F |
|
NA | 32.4 ± 5.9 | 131.0 ± 2.3 |
| 21f | -F |
|
NA | 79.6 ± 3.9 | 146.0 ± 4.5 |
| 21g | -F |
|
21.4 ± 3.7 | 134.0 ± 4.2 | 147 ± 5.4 |
| 23 | -F |
|
31.4 ± 2.9 | 79.9 ± 3.1 | 85.6 ± 1.9 |
| 26a | -F |
|
NA | 15.4 ± 1.7 | 88.6 ± 2.1 |
| 26b | -F |
|
2.0 ± 2.3 | 0.9 ± 2.1 | 64.2 ± 5.0 |
| 30a | -F |
|
50.6 ± 3.3 | 104.0 ± 1.0 | 123.0 ± 1.7 |
| 30b | -F |
|
NA | NA | 30.7 ± 4.9 |
| 30c | -F |
|
NA | NA | 86.6 ± 8.7 |
| 30d | -F |
|
NA | NA | 84.6 ± 5.3 |
a
Cell death percentage was normalized to control (0 %) and E2 (100 %) assessed in a 9 day cell viability assay with drug treatments renewed every 3 days. Data represented as mean ± s.e.m. is an average of at least three cell passages (triplicates in each passage).