Table 1.
Primers Used for RT-PCR and Nucleotide Sequencing
| Primer name | Primer sequence (5′→3′) | Target gene, virus | Amplicon length (bp) | Used for |
|---|---|---|---|---|
| FU2new | GCCGATGACGTAGCCGGTTGGGATAC | NS5, Flavivirus genus | 888 | Sequencing |
| cFD3 | AGCATGTCTTCCGTGGTCATCCA | NS5, Flavivirus genus | ||
| ISFfwd | GGATTATCTGGTACATGTGG | NS5, ISFs | 518 | Sequencing |
| CSFLAVrev | TAAGCCAGGCGTCAATCTCT | NS5, CsFV | ||
| CSFLAVfwd | CCGTACCACAGGAAACTCGT | NS5, CsFV | 255 | Virus detection |
| CSFLAVrev | TAAGCCAGGCGTCAATCTCT | NS5, CsFV | ||
| CSLfwd | CGAGTACGAGGCTCTAGGGT | NS5, CsFV | 913 | Sequencing, virus |
| CSLrev | GACCAGTTGGCATCCACTCA | NS5, CsFV | confirmation |
ISF, insect-specific flavivirus; CsFV, Culiseta flavivirus.