FIG. 2.

Analysis of genetic variability in protease sequences. To analyze the variability in the protease gene, we performed Shannon entropy analysis on patient-derived protease sequences. The Shannon entropy analysis revealed that the region of protease spanning nucleotides 1–18, 28–57, 63–67, 74–80, 82–89, 98–115, 120–140, 145–160, and 205–235 exhibited high genomic variability (highlighted gray). The high entropy areas highlighted in dark gray show the region of protease (spanning nucleotides 1–90; HBX2 amino acids 20–50) where certain major PI resistance mutations tend to occur more frequently.