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Pseudovirion incorporation of Env. Incorporation of Env was measured by ultracentrifugation of C18, C63, and C63-R658K pseudoviruses followed by Western blotting after normalizing p24 levels. The Western blot was probed with sheep anti-gp120 and rabbit ARP432 raised against gp120 and p24, respectively. The positive control (+) of gp120 was HIV-1 gp120 CM Envelope protein (Cat. #2968) and p24 was HIV-1 p24 IIB (Cat. #12028) (NIH Aids Reagent Program).
