Table 1.
Sequences of Du151 Envelope Clones, Chimeras, and Mutants Showing gp41 Amino Acid Residues That Differ Between Clones C63 and C18
| mpi | Clone ID | Abbreviation | Virus | Position 658 | Frequencya | Position 671 | Frequency | Position 717 | Frequency |
|---|---|---|---|---|---|---|---|---|---|
| 1–2 | Du151_1A_C1 | C1 | A | QQEKNEKDLLALD | 12/12 | SWKNLWNWFNITN | 12/12 | GYSPLSFQTLTPS | 12/12 |
| 8 | Du151_8A_C9 | C9 | A/B | ……K…… | 6/6 | ……N..D… | 6/6 | ……F…..N | 1/6 |
| 19 | Du151_19AB_C63 | C63 | A/B | …Q..R…… | 1/11 | K.Q…S…… | 10/11 | ……L…..N | 6/11 |
| 19 | Du151_19AB_C18 | C18 | A/B | …Q..K…… | 10/11 | K.Q…G…… | 1/11 | ……F…..N | 5/11 |
| 19 | C63-gp120/C18-gp41 | C18-gp41 | A/B | …Q..K…… | K.Q…G…… | ……F…..N | |||
| 19 | C18-gp120/C63-gp41 | C18-gp120 | A/B | …Q..R…… | K.Q…S…… | ……L…..N | |||
| 1–2 | Du151_1A_C1_K658R | C1-K658R | A | QQEKNERDLLALD | SWKNLWNWFNITN | GYSPLSFQTLTPS | |||
| 8 | Du151_8A_C9_ K658R | C9-K658R | A/B | ……R…… | ……N.D.. | ……F…..N | |||
| 19 | Du151_19AB_C18_ K658R | C18-K658R | A/B | …Q..R…… | K.Q…G…… | ……F…..N | |||
| 19 | Du151_19AB_C63_ R658K | C63-R658K | A/B | …Q..K…… | K.Q…S…… | ……L…..N | |||
| 19 | Du151_19AB_C63_ S671G | C63-S671G | A/B | …Q..R…… | K.Q…G…… | ……L…..N | |||
| 19 | Du151_19AB_C63_ L717F | C63-L717F | A/B | …Q..R…… | K.Q…S…… | ……F…..N |
a
The frequency of each amino acid (highlighted in bold) is indicated as the number of clone sequences carrying that specific residue relative to total clones at each time point. The 2F5 (ALDSWKN) and 4E10 (NWFNIT) epitopes are underlined.