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. 2016 Mar 7;6:22721. doi: 10.1038/srep22721

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Vero cells, grown on glass coverslips, were cooled to 4 °C and incubated for 30 min with ricin-FITC. The cells were then washed, treated with naïve (control), anti-ricin and anti-RiVax polyclonal mouse sera for an additional 30 min at 4 °C and then shifted to 37 °C. At the indicated time points (90 min and 4 hr) the cells were fixed and imaged by confocal microscopy. Insets in the right and left hand columns highlight the subcellular localization of ricin (+naïve sera, white arrowheads) and ricin-sera complexes (black arrowheads). Scale bar, 5 μm.