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. 2016 Mar 7;6:22252. doi: 10.1038/srep22252

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Copyright © 2016, Macmillan Publishers Limited

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(a) pFGS, (b) pEGS, (c) pFGF, (d) pFA7. PcFCPpro, Pleurochrysis carterae FCP promoter; PyGUS, Pyropia yezoensis GUS gene; NOSter, nopaline synthase terminator derived from pBI221 vector; PcEF-1αpro, P. carterae EF-1α promoter; sGFP, a synthetic GFP gene; PyAph7, a hygromycin B-resistant gene optimized for P. yezoensis; CrRbcSter, Chlamydomonas reinhardtii ribulose 1,5-bisphosphate carboxylase/oxygenase small subunit terminator. *Twenty base pairs derived from the pGEM-T Easy vector were inserted. Arrows in (d) indicate a primer set used in the genomic PCR analysis. The double-headed arrow in (d) indicates the probe used in Southern hybridization analysis.