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. 2016 Mar 7;6:22252. doi: 10.1038/srep22252

Figure 4. Antibiotic selection and gene expression of mutant strains.

Figure 4

(a) Intact cells (left) and pFA7-introduced cells (right) after 3 weeks of selection on hygromycin B (2.5 mg/mL)-containing SLEP (representative image of n = 4). Arrows indicate the cells undergoing mitosis. Scale bars, 50 μm. (b) pFA7-introduced cells (upper panels) and intact cells (lower panels) on the hygromycin B-containing selective plates after 4 weeks of selection. Brown colonies were observed only in the transformed groups (representative image of n = 4). (c) Genomic PCR for ORF of endogenous FCP (upper), and for a region including the 3′-end of the FCP promoter and the 5′-end of the PyAph7 ORF derived from pFA7 (lower) in the ten transformed mutant strains (1–10) and wild-type strain (WT); M, molecular marker. (d) Expression of FCP (upper) and PyAph7 (lower) in the ten transformed mutant strains (1–10) and wild-type (WT) strain; M, molecular marker. (e) Southern hybridization for seven mutant strains and a WT strain; molecular marker bands are on the left.