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. 2016 Mar 7;6:22780. doi: 10.1038/srep22780

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Representative images (A) and summarized data (B) showing TRPV4 expression levels in cultured primary MAECs obtained from young and aged rats and evaluated using Western blotting assays. β-tubulin was used as a loading control. Protein levels are expressed as relative optical densities. Mean ± SE (n = 3). ^*P < 0.05, young (3 months old) vs. aged (22 months old) rats. (C) MAECs are immunostained with an anti-TRPV4 antibody. In contrast to the control (omission of primary antibody), immunostaining for TRPV4 is detected in MAECs from young and aged rats.