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. 2016 Mar 7;7:23. doi: 10.3389/fneur.2016.00023

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Copyright © 2016 Thelin, Frostell, Mulder, Mitsios, Damberg, Aski, Risling, Svensson, Morganti-Kossmann and Bellander.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence of the analyzed proteins. The image illustrates representative immunofluorescence to the following proteins: (A) HIF-1α (HIF) (day 1), (B) VEGF (day 1), (C) C5b-9 (day 1), (D) IgG (day 1), (E) Activated Caspase 3 (day 1), (F) Granulocytes (CD43, day 1), (G) Proliferating microglia (CD34, day 7), and (H) Macrophages (ED1, day 7) (all red, respectively) at 10× amplification from normoxic animals. NeuN was used to label neurons (green). DAPI was used as counterstaining for all cells (blue). All pictures are taken from the peri-lesional area; the cavity is located to the right of each picture. Scale bar = 100 μm.