Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2016 Nov 1.

Published in final edited form as: J Mol Cell Cardiol. 2015 Sep 24;88:73–81. doi: 10.1016/j.yjmcc.2015.09.005

(a) IPA core analysis was used to functionally classify proteins mapped from peptides that were detected only Sirt5^−/− samples. (b) A label free analysis program (QUOIL) was used to determine the relative abundance of succinylated peptides detected in both WT and Sirt5^−/− samples. Represented here are the 16 peptides from 9 proteins increased by > 30% with p < 0.05 in Sirt5^−/− hearts. (c) Relative protein quantitation was calculated based on the intensities of TMT report ions using Scaffold 4.0 (Proteome Software, Portland, OR). The average ratio of protein expression (Sirt5^−/−: WT) is expressed numerically. The data demonstrate no significant differences in total protein abundance between Sirt5−/− and WT mice. (d) Conservation of the Sirt5 substrate lysines across human, mouse, rat, and S. Cerevisiae (yeast). Protein alignments were performed with the “Align” tool at www.uniprot.org; “+” indicates a conserved lysine, “−“ indicates that the lysine is not conserved, “n.h.” indicates that a homologous protein is not expressed by the organism.

(a) IPA core analysis was used to functionally classify proteins mapped from peptides that were detected only Sirt5^−/− samples. (b) A label free analysis program (QUOIL) was used to determine the relative abundance of succinylated peptides detected in both WT and Sirt5^−/− samples. Represented here are the 16 peptides from 9 proteins increased by > 30% with p < 0.05 in Sirt5^−/− hearts. (c) Relative protein quantitation was calculated based on the intensities of TMT report ions using Scaffold 4.0 (Proteome Software, Portland, OR). The average ratio of protein expression (Sirt5^−/−: WT) is expressed numerically. The data demonstrate no significant differences in total protein abundance between Sirt5−/− and WT mice. (d) Conservation of the Sirt5 substrate lysines across human, mouse, rat, and S. Cerevisiae (yeast). Protein alignments were performed with the “Align” tool at www.uniprot.org; “+” indicates a conserved lysine, “−“ indicates that the lysine is not conserved, “n.h.” indicates that a homologous protein is not expressed by the organism.