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STD displacement titrations of (A) acetylcholine using the choline N(CH2) resonance (choline concentration of 100 μM and ct-AChBP concentration of 5 μM), (B) nicotine using 100 μM acetylcholine as the NMR reporter ligand, and (C) α-bungarotoxin (BTX) using 50 μM nicotine as the NMR reporter ligand. NMR studies were performed at 25 °C.
