Table 3.
Primary and secondary antibodies used in immunohistochemistry experiments.
| Antigen | Code | Host | Dilution | Supplier |
|---|---|---|---|---|
| PRIMARY ANTIBODIES | ||||
| Connexin 43 (Cx43) | ab11370 | Rabbit (rb) | 1:700 | Abcam |
| Neurofilament 160 (NF-160) | ab7794 | Mouse (ms) | 1:1000 | Abcam |
| Vimentin | M0725 | Mouse (ms) | 1:150 | Dako |
| Adenosine receptor A1 | AB 1587P | Rabbit (rb) | 1:100 | Chemicon |
| Muscarinic receptor M2 | AMR-002 | Rabbit (rb) | 1:200 | Alomone |
| Small-conductance Ca2+-activated K+ channel (KCa2.2) | AP10032PU-N | Goat (gt) | 1:400 | Acris antibodies |
| Small conductance Ca2+-activated K+ channels (KCa2.3) | ab83737 | Rabbit (rb) | 1:300 | Abcam |
| High voltage-activated Ca2+ (L-type) channels (Cavα2δ−1) | ab2864 | Mouse (ms) | 1:50 | Abcam |
| G protein-coupled inwardly rectifying K+ channels (KIR3.1) | ab61191 | Rabbit (rb) | 1:500 | Abcam |
| SECONDARY ANTIBODIES | ||||
| Alexa Fluor 488 anti-rb | A-21206 | Donkey | 1:1500 | Molecular probes |
| Alexa Fluor 568 anti-ms | A-10037 | Donkey | 1:1500 | Molecular probes |
| Alexa Fluor 568 anti-gt | A-11057 | Donkey | 1:1500 | Molecular probes |