Figure 7. Relative expression of the genes putatively involved in xyloglucan utilization in R. cellulolyticum grown on different substrates.
Total RNAs were prepared from cultures on cellobiose, cellulose, xyloglucan, and XGO4 generated from xyloglucan by digestion with Cel9X, and reverse transcribed. The PCR were then performed with the primer pairs listed in supplemental Table S4. For each gene the relative expression on a given growth substrate versus cellobiose is given after standardization with 16S rRNA encoding gene amplifications. The data show the mean and standard errors from three independent biological replicates. The loci numbers, the predicted functions of the gene products and the schematic representation of the divergent clusters of interest are as in Fig. 6.