Table 1. Specific activities of wild type and engineered cellulosomal enzymes on cellulosic substrates and xyloglucan.
| Substrate (concentration) | Enzyme |
||||
|---|---|---|---|---|---|
| Cel9Xa | Cel9Ua | Cel44O | 9X-44O | Xgh74A | |
| CMC (10 g/L) | – | 6,666 ± 310 | 2,613 ± 627b | 2,584 ± 511 | 1,057 ± 7 |
| PASC (3.5 g/L) | – | 736 ± 6.5 | 393 ± 96 | 413 ± 48 | 55 ± 3.5 |
| Avicel (3.5 g/L) | – | 44.9 ± 0.4c | 38 ± 2 | 31 ± 0.3 | 10.7 ± 0.7 |
| Xyloglucan (3.5 g/L) | 1,250 ± 85 | 1,069 ± 243 | 922 ± 95 | 1,340 ± 14.4 | 2,160 ± 126 |
aThe data are from ref. 19.
bThe data show the mean and standard deviation of 2 to 4 replicates.
cValues are given in μmol of products released per μmol of enzyme × min−1, except for Avicel where the values are in μmol of products released after 24 h of incubation with 0.1 μmol of enzyme.