Figure 2.

Effect of Erl/GLE in cell motility and cell aggregation. Quiescent SUM-149 cells were seeded on top chambers and treated with vehicle, 0.1 µM-Erlotinib, 0.05 mg/mL-GLE or Erl/GLE for 72h. A, B. Cells migrated or invaded towards 10%FBS medium. Cells were fixed and stained, micrographs were captured, and cells quantified (ImageJ). C. SUM-149 cells were seeded on MatTek dishes, labeled and overlaid with Matrigel. Next day, cells were treated as above. Micrographs were obtained with a 20x objective magnification at the same location. These represent the average of 10 photos taken by treatment. Scale bars = 100µm. Columns represent means ± SEM. Experiments were repeated at least three times. Significance against: vehicle (*), Erlotinib (ᶲ) and GLE (ᵟ). (P≤0.05).