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. 2016 Feb 5;7(5):500–511. doi: 10.7150/jca.13599

Figure 3.

Figure 3

Effect of Erl/GLE in BC cells with acquired and intrinsic Erlotinib resistance. A, B. rSUM-149 cells were treated with Erl, GLE or Erl/GLE for 72h and cell viability and CIs were calculated based on the IC50 of each at a constant Erl:GLE (1:24) and at NCR (Erl+0.2 mg/mL-GLE). C. rSUM-149 cells were seeded in 3D (see Fig.2) and treated with vehicle, 2.0 µM-Erlotinib, 0.2 mg/mL-GLE or Erl/GLE for 72h. Micrographs were obtained with a 20x objective magnification at the same location. These represent the average of 10 photos taken by treatment. Scale bars = 100µm. D, E. MDA-MB-231 cells were treated with Erl, GLE or Erl/GLE for 72h and cell viability and CIs were calculated based on the IC50 of each at a constant Erl:GLE (1:47) and at NCR (Erlotinib+0.2 mg/mL-GLE). Columns represent means ± SEM. Experiments were repeated at least three times. Significance against: Erlotinib (*) or GLE (ᶲ) (P≤0.05).