Figure 5.

In vivo Erl/GLE effect. 1x10⁶ SUM-149 cells mixed with Matrigel (1:1) were injected into the mammary fat pad of SCID mice. Mice were gavaged daily with vehicles (open triangles and open diamonds) or experimental groups (closed circles and closed squares for 13wks. A. Weekly mouse weight. B. Weekly tumor volume. C. Average tumor volume. D. Final tumor weight. E, F. Immunoblot with the primary antibodies listed on the left side of the panel, β-actin is loading control. Densitometric analyses were done using Image J software. Data is expressed as phospho/total protein ratio. Each lane represents a different animal. Estimated marginal means ± SEM. Significance against: (*) vehicle Erl+GLE, (ᵟ) vehicle Erlotinib. P≤0.05.