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. 2016 Mar 7;6:22675. doi: 10.1038/srep22675

Figure 4. Characterization of functionalized antibody using electrospray ionization time-of-flight (ESI-TOF) mass spectrometry and LC-MS/MS.

Figure 4

(a) Western blot of functionalized antibody (anti-TGM1) and conjugation with TCO-PEG5000. (b,c) Non-functionalized and functionalized mouse antibody (anti-TGM1) analysed by ESI-TOF. (d) Overview functionalized lysine residues and peptides of functionalized anti-TGM1 antibody analysed by LC-MS/MS. (e) Mapping of functionalized residues on mouse IgG2a. Structure and amino acid numbering based on PDB file 1IGT. HC = heavy chain, LC = light chain.