Fig. 3.

Electrophoresis banding pattern of PCR product 1 after amplification with primers aflR-1 and aflR-2 (A) and PCR product 2 after the nested PCR amplification with primers aflR-1a and aflR-2b (B). (A) Lane M, 100 bp DNA ladder standard; AF, positive control; a, b & c, sample groups A, B & C respectively. The amplified DNA fragment sizes (800 bp) were estimated after comparison with a commercial 100 bp DNA ladder. (B) Lane M, 100 bp DNA ladder standard; a1–a4, b1–b4, c1–c4, sample groups A, B & C respectively. The amplified DNA fragment sizes (400 bp) were estimated after comparison with a commercial 100 bp DNA ladder.