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. 2016 Jan 31;32(1):81–87. doi: 10.5487/TR.2016.32.1.081

Fig. 4.

Fig. 4

Electrophoresis banding pattern after PCR product 1 (A), PCR product 2 (B) and double (C) digestions with Hinc II and Pvu II. (A) Lane M, 100 bp DNA ladder standard; AF, positive control (4 PCR-based detection); A1, B1 & C1, products of digestion with Pvu II (4 PCR-based detection); A2, B2 & C2, products of digestion with Hinc II (5 PCR-based detection); UNCUT, native PCR product. (B) Lane M, 100 bp DNA ladder standard; A1, B1 & C1, products of digestion with Pvu II (3 PCR-based detection); A2, B2 & C2, products of digestion with Hinc II (2 PCR-based detection). (C) Lane M, 100 bp DNA ladder standard; T4 & T7, products of digestion with Pvu II; T1, T3 & T6, products of digestion with Hinc II. The digestion conditions were essentially the same as for single digests, except that 20 units of Hinc II and 40 units of Pvu II were used in 2 × concentrated buffer, T8 and T9 (2 PCR-based detection).