Table 1.

The reaction mixture for amplifying DNA extracted from maize samples

Mixture	Ref.	T1	T2	T3	T4	T5	T6	T7	T8	T9
10xbuffer	2.5 μL	2.5 μL	2.5 μL	2.5 μL	2.5 μL	2.5 μL	2.5 μL	2.5 μL	2.5 μL	2.5 μL
dNTP	4 μL	4 μL	4 μL	4 μL	4 μL	4 μL	4 μL	4 μL	4 μL	4 μL
Primer F	0.5 μL	1 μM	1 μM	1 μM	1 μM	5 μM	5 μM	10 μM	10 μM	20 μM
Primer R	0.5 μL	1 μM	1 μM	1 μM	1 μM	5 μM	5 μM	10 μM	10 μM	20 μM
Taq DNA	0.5 μL	0.5 μL	0.5 μL	0.5 μL	0.5 μL	0.5 μL	0.5 μL	0.5 μL	0.5 μL	0.5 μL
Template	1 μL	1 μL	2 μL	3 μL	5 μL	1 μL	2 μL	1 μL	2 μL	2 μL
Dist.water	16 μL	16 μL	15 μL	14 μL	12 μL	16 μL	15 μL	16 μL	15 μL	15 μL
Total	25 μL	25 μL	25 μL	25 μL	25 μL	25 μL	25 μL	25 μL	25 μL	25 μL

Ref., Reference standard; T1–T9, different PCR conditions; Taq DNA, Taq DNA polymerase; Dist. water, Distilled water.