Table 2.

PCR amplification reactions for PCR programs 1 and 2

PCR cycle	Program 1	Program 2
Pre-denaturation	95°C, 10 min	94°C, 5 min
Denaturation	94°C, 30 sec	94°C, 30 sec
Annealing	50°C, 45 sec	50°C, 1.25 min
Extension	72°C, 1.15 min	72°C, 1.40 min
Final extension	72°C, 10 min	72°C, 10 min