Table 3.

The oligonucleotide primers used for conventional PCR

No	Primers	Gene	Primer sequence (5′-3′)	Amplicon size (bp)
1	First PCR	aflR-1 aflR-2	AAC CGC ATC CAC AAT CTC AT AGT GCA GTT CGC TCA GAA CA	800 bp
2	Nested PCR	aflR-1a aflR-2b	GCA CCC TGT CTC CCC TAA CA ACG ACC ATG CTC AGC AAG TA	400 bp

The first primers aflR-1 and aflR-2 and the nested primers aflR-1a and aflR-2b were used.