Table 4.
The oligonucleotide primer pairs used for SYBR green real-time PCR amplifications
| No | Primer pairs | Gene | Primer sequence (5′-3′) | Annealing temperature |
|---|---|---|---|---|
| 1 |
Tub1-F Tub1-R |
tub1 | GTC CGG TGC TGG TAA CAA CT GGA GGT GGA GTT TCC AAT GA |
65 |
| 2 |
Ver1 Ver2 |
aflM | GCC GCA CGC GGA GAA AGT GGT GGG GAT ATA CTC CCG CGA CAC AGC C |
65 |
| 3 |
aflR 660 aflR 1249 |
aflR | CGC GCT CCC AGT CCC CTT CAT T CTT GTT CCC CGA GAT GAC CA |
59 |
| 4 |
nor1 nor2 |
aflD | ACC GCT ACG CCG GCA CTC TCG GCA C GTT GGC CGC CAG CTT CGA CAC TCC G |
65 |
The oligonucleotide primer pairs were used to target the specific fungal genes: Tub1-F and Tub1-R for tub1; Ver1 and Ver2 for aflM (versicolorin A); aflR 660 and aflR 1249 for aflR; nor1 and nor2 (norsolorinic acid) for aflD.