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. Author manuscript; available in PMC: 2016 Mar 7.
Published in final edited form as: Methods Mol Biol. 2014;1102:679–695. doi: 10.1007/978-1-62703-727-3_37

Fig. 3.

Fig. 3

Frozen tissue preparation. (a) Example of liquid nitrogen cryomold freezing stand (LN2 Stand). (b) LN 2 stand in ice bucket with liquid nitrogen. (c) Examples of: OCT compound; labeled cryomold; and prepped tissue ready for freezing (not on ice; for demonstration purposes only). (d) Process of rapid freezing of tissue in OCT (partially hardened) using LN2 stand. (e) Process of rapid freezing of tissue in OCT (fully hardened) using slurry method (see Note 15). (f) Example of labeled aluminum foil OCT archival sample wrapper. (g) Example of frozen OCT sample just prior to wrapping. (h) Examples of wrapped sample (1) backside and (2) front side. (i) Example of wrapped labeled sample placed in archival cryobox