Figure 3. Translation suppression in MCF7 cells caused by RNA G-quadruplexes located in the 5′ UTR and ORF.

(a) Translated products from reporter mRNAs detected by western blotting. (b) Translation efficiencies of 5′ UTR reporter (blue) and ORF reporter (red) mRNAs. The values were calculated by dividing relative R/F protein ratio by relative R/F mRNA ratio (see Figs S2b and S3, in the Supporting Information).