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. 2016 Mar 7;3:7. doi: 10.3389/fnut.2016.00007

Figure 4.

Figure 4

Dolichos biflorus agglutinin (DBA)-rhodamine in C. elegans co-localized with GFP-DAergic neurons of aged and young nematodes (L3). GFP-DAergic neurons in aged animals (A) or young animals (D). DBA-rhodamine in GFP-DAergic neurons in aged animals (1.0 × 10−3 or 3.33 × 10−3 mM) (B) or young animals (E). Merged (A,B) showing co-localization of the DBA-rhodamine with GFP-DAergic neurons in aged animals (C) or young animals (F). (G) The number of DAergic neurons was not altered (7.5, n = 5, P > 0.2) and 50% of them were co-localized with DBA-rb (1.0 × 10−3 or 3.33 × 10−3 mM, n = 3) in the aged animals. (H) The fluorescence intensity per GFP-DAergic neuron. (I) The size of the GFP-DAergic neurons was not changed. These observations were similarly seen in young animals. (J) The APS was increased at lower doses of DBA, and decreased at higher doses. (K) Mean survival time of each group. *indicates statistical significance.

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