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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Virology. 2015 Aug 21;485:492–501. doi: 10.1016/j.virol.2015.07.018

Fig.2.

Fig.2

Schematic of the PV1 RNA transcripts used in this study. (A) PV1(A)80 transcript RNA contains the entire poliovirus genomic RNA sequence. This RNA encodes all of the viral proteins and serves as a template for (−) strand synthesis. (B) P23 transcript RNA contains a deletion of the P1 capsid coding region, but encodes the viral replication proteins and serves as a replicon RNA. Mutations constructed in 3C/3D/3CD are indicated by arrows and include the 3C-RNA binding mutant (RBM; K12N/R13N), the 3CD processing mutant (PM; T181K, Q182D, S183G, Q184N), and the 3D polymerase activity mutant (G327M). (C) The viral protein expression RNA contains the specific protein coding sequence flanked by the authentic PV1 5’ NTR, IRES and 3’ NTR and poly(A) tail.